Development and Use of Anucleate Bacterial Cells to Assay the in vitro Activity of Pollutants

The T7 0.3 gene product 0.3 protein was purified by a modification of the published procedure 2, and used to raise antibody to this protein. A radioimmune precipitation RIP assay was developed which could be used to estimate the increased misincorporation of cysteine into 0.3 protein. Parameters of the RIP assay were varied to make the RIP-polyacrylamide gel electrophoresis RIP-PAGE assay specific for the 0.3 protein. A single protein band was, however, never achieved although increased misincorporation of cysteine into the 0.3 protein can now be estimated by RIP-PAGE combined with scanning densitometry.