Identification of Leukocyte Subpopulations Responsible for the Production of Osteoclast Activating Factor and their Role in Bone Resorption.

reportActive / Technical Report | Accession Number: ADA093782 | Open PDF

Abstract:

Osteoclast Activating Factor OAF was predictably produced in large volume mitogen stimulated MNC cultures. OAF production was independent of DNA synthesis. OAF was produced in mixed leukocyte cultures containing heat inactivated fetal calf serum. OAF production was independent of blastogenesis. OAF activity was isolated in the molecular weight range of 12,000 to 25,000 daltons by S-200 Sephacryl chromatography and high performance liquid chromatography HPLC. OAF activity was concentrated across membranes and by ammonium sulfate precipitation. Subsequent HPLC produced two peaks of bone resorbing activity a major peak at 18,000 daltons and a minor peak at 9,000 daltons. MNC culture supernatants also contained high concentrations of low molecular weight prostaglandins which also are believed to be osteolytic in vitro. Author

Author(s):
Hawley, Charles E.
Author Organization(s):
ARMY INST OF DENTAL RESEARCH WASHINGTON DC
Descriptive Note:
Final rept. Oct 77-Sep 80,
Pagination:
0038

Security Markings

DOCUMENT & CONTEXTUAL SUMMARY

Distribution:
Approved For Public Release

RECORD

Collection: TR
Identifying Numbers
Report Number(s):
OG-6039
 Subject Terms
Joint Capability Areas:
JCA_8_Building Partnerships; JCA_8.1.1_Inform Domestic and Foreign Audiences; JCA_8.1_Communicate; JCA_1.4_Force Support; JCA_1.4.1_Force Health Protection; JCA_5_Command and Control; JCA_1_Force Support; JCA_6.4.1_Secure Information Exchange; JCA_6_Net Centric; JCA_1.2_Force Preparation; JCA_5.3_Planning; JCA_3_Force Application; JCA_6.4_Information Assurance; JCA_1.2.7_Experimentation; JCA_1.3_Human Capital Management; JCA_3.2_Engagement; JCA_1.2.3_Educating; JCA_1.3.2_Personnel Management; JCA_1.4.2_Health Service Delivery; JCA_3.2.1_Kinetic Means; JCA_4.2.2_Inventory Management
Modernization Areas:
Fully Networked C3
Communities of Interest:
No COI(s) Identified
Descriptor(s):
*LEUKOCYTES, PRODUCTION, LITERATURE SURVEYS, IDENTIFICATION, BIOLOGICAL ABSORPTION, BONES
Field(s)/Group(s):
Medicine and Medical Research
Keyword(s):
Osteoclast activating factor
Report Date:
1981 Dec 31