Correction of Quenching Errors in Analytical Fluorimetry through Use of Time Resolution.

reportActive / Technical Report | Accession Number: ADA085130 | Open PDF

Abstract:

Varying concentrations of quenching agents can cause serious errors in analytical fluorimetry. The origin of these errors is an unexpected change in the quantum efficiency for the observed luminescence. In this paper, it is recalled that quantum efficiency can be expressed as the ratio of an observed luminescence decay time to the decay time which would be observed in the absence of quenchers. Because this latter quantity is a constant for any particular fluorophore, quantum efficiency variations can be compensated through measurement of the decay time. For such measurements, the time-correlated single photon technique was employed and measured luminescence values were taken both from averaged photon count rates and from integrated fluorescence decay plots. Division of these values by measured luminescence lifetimes produced values which were independent of quencher concentration. Systems studied were quinine bisulfate quenched with chloride ion and 1-pyrenebutyric acid quenched by iodide. Author

Author(s):
Hieftje, Gary M. ; Haugen, Gilbert R.
Author Organization(s):
INDIANA UNIV AT BLOOMINGTON DEPT OF CHEMISTRY
Descriptive Note:
Technical rept.,
Pagination:
0021

Security Markings

DOCUMENT & CONTEXTUAL SUMMARY

Distribution:
Approved For Public Release

RECORD

Collection: TR
Identifying Numbers
Report Number(s):
TR-25, 32
Contract/Grant Number(s):
N00014-76-C-0838, PHS-GM-24473
 Subject Terms
Joint Capability Areas:
JCA_1.2.3_Educating; JCA_5_Command and Control
Modernization Areas:
Quantum Science and Computing
Communities of Interest:
Biomedical
Descriptor(s):
*FLUORESCENCE, *QUENCHING, *QUANTUM EFFICIENCY, *CHEMICAL ANALYSIS, IONS, PHOTOCHEMICAL REACTIONS, ERRORS, CHLORIDES, SULFATES, FLUOROMETERS, PHENANTHRENES, DECAY SCHEMES, IODIDES, QUININE, BUTYRIC ACIDS
Field(s)/Group(s):
Organic Chemistry, Radiation and Nuclear Chemistry
Keyword(s):
Fluorimetry, Time resolution, WUNR051622
Report Date:
1980 May 27