Experiments and Simulations on Short Chain Fatty Acid Production in a Colonic Bacterial Community

reportActive / Technical Report | Accession Number: AD1096837 | Open PDF

Abstract:

Understanding how production of specific metabolites by gut microbes is modulated by interactions with surrounding species and by environmental nutrient availability is an important open challenge in microbiome research. As part of this endeavor, this work explores interactions between F. prausnitzii, a major butyrate producer, and B. thetaiotaomicron, an acetate producer, under three different in vitro media conditions in monoculture and coculture. In silico Genome27scale dynamic flux balance analysis dFBA models of metabolism in the system using COMETS Computation of Microbial Ecosystems in Time and Space are also tested for explanatory, predictive and inferential power.

Author(s):
Yu, Bea ; Dukovski, Ilija ; Kong, David ; Bobrow, Johanna ; Ostrinskaya, Alla ; Segre, Daniel ; Thorsen, Todd
Author Organization(s):
MIT Lincoln Laboratory Lexington United States
Descriptive Note:
Journal Article - Open Access
Supplementary Note:
bioRxiv.org , 01 Jan 0001, 01 Jan 0001,
Pagination:
0035

Security Markings

DOCUMENT & CONTEXTUAL SUMMARY

Distribution:
Approved For Public Release
Distribution Statement:
Approved For Public Release;

RECORD

Collection: TR
Identifying Numbers
Contract/Grant Number(s):
FA8702-15-D-0001
 Subject Terms
Joint Capability Areas:
JCA_5_Command and Control; JCA_5.3_Planning; JCA_1.2.7_Experimentation; JCA_1.2.5_Lessons Learned; JCA_4.6_Engineering; JCA_1.2.3_Educating; JCA_5.2.2_Develop Knowledge and Situational Awareness; JCA_5.2_Understand
Modernization Areas:
Biotechnology
Communities of Interest:
Biomedical
Descriptor(s):
mass spectrometry, electrospray ionization, culture techniques, analysis of variance, measurement, fatty acids, statistical analysis, microorganisms, experimental data, bacteria, METABOLITES
Field(s)/Group(s):
Microbiology
Keyword(s):
short chain fatty acid production
Report Date:
2018 Oct 16
Creation Date:
2020 Apr 24