Apyrase: A Portable Treatment to Prevent Burn Progression and Infection

Year one focused on administrative and regulatory requirements, initiation of experimentation, assessment of techniques, and analysis of preliminary results. This laid the ground work for subsequent experimentation. IACUC and ACURO approvals were obtained in the first quarter, along with approval for Specific Aim 2 bacterial studies. In vivo experimentation began in the third quarter and was continued in the fourth quarter. Methods for anesthesia, analgesia and bandaging were confirmed and standardized. The parameters for burn wounds duration of contact and placementspacing. The randomization and the delivery method for the test agents were determined. Sample collection strategies were confirmed. Sample collection, processing and analysis began in the fourth quarter. This includes processing of the histology samples and swabs for ATP assays taken at each time point for each animal. Photographs of each wound over the course of the experiment have been archived. Our goal was to have all of the animal experimentation for the first specific aim completed in the first year.