Development of a Liquid Chromatography High Resolution Mass Spectrometry (LC-HRMS) Method for the Quantitation of Viral Envelope Glycoprotein in Ebola Virus-Like Particle Vaccine Preparations

reportActive / Technical Report | Accession Number: AD1021613 | Open PDF

Abstract:

Ebola virus like particles EBOV VLPs, eVLPs, are produced by expressing the viral transmembrane glycoprotein GP and the structural matrix protein VP40 in mammalian cells. When expressed, these proteins self-assemble and bud from host cells displaying morphology similar to infectious virions. Several studies have shown that rodents and non-human primates vaccinated with eVLPs are protected from lethal EBOV challenge. The mucin-like domain of envelope glycoprotein GP1 serves as the major target for a productive humoral immune response. Therefore GP1 concentration is a critical quality attribute of EBOV vaccines and accurate measurement of the amount of GP1 present in eVLP lots is crucial to understanding variability in vaccine efficacy.

Author(s):
Cazares, Lisa H. ; Ward, Michael D. ; Brueggemann, Ernie ; Kenny, Tara ; Demond, Paul ; Mahone, Chris ; Martins, Karen A. ; Nuss, Jonathan E. ; Glaros, Trevor ; Bavari, Sina
Author Organization(s):
USAMRIID Frederick United States
Descriptive Note:
OSTP Journal Article
Supplementary Note:
Clinical Proteomics , 13, 1, 01 Jan 0001, 01 Jan 0001,
Pagination:
0044

Security Markings

DOCUMENT & CONTEXTUAL SUMMARY

Distribution:
Approved For Public Release
Distribution Statement:
Approved For Public Release;

RECORD

Collection: TR
Identifying Numbers
Report Number(s):
TR-16-141
Project Number(s):
1325119
 Subject Terms
Joint Capability Areas:
JCA_5_Command and Control; JCA_5.3_Planning; JCA_1.2.7_Experimentation
Modernization Areas:
Biotechnology
Communities of Interest:
No COI(s) Identified
Descriptor(s):
liquid chromatography, mass spectrometry, high resolution, GLYCOPROTEINS, vaccines, HUMORAL IMMUNITY, PEPTIDES
Field(s)/Group(s):
Pharmacology, Biochemistry, Medicine and Medical Research
Keyword(s):
lc hrms (Liquid Chromatography high resolution mass spectrometry), stable isotope dilution quantitation, ebov vlp (Ebola virus like particles), evlp (ebov vlp), gp (glycoproteins), mld (mucin like domain)
Report Date:
2016 Sep 05
Creation Date:
2017 Jun 27