The Expression of Fos, Jun and AP-1 DNA Binding Activity in Rat Supraoptic Nucleus Neurons Following Acute Versus Repeated Osmotic Stimulation

reportActive / Technical Report | Accession Number: AD1011468 | Open PDF

Abstract:

FOB and Jun are protein products of their respective proto-oncogenes, c-fos and c-jun. Nuclear concentrations of both proteins rapidly, but transiently, rise after thepresentation of a variety of stimuli under in vitro and in vivo conditions. Fos and Jun can form heterodimeric proteins that act as transcription factors to regulate late response genes.Previous work has demonstrated that hypertonic saline injection results in Fos expression in supraoptic nucleus SON neurons that mediate body water homeostasis. Since Fos is thought to act mostly in conjunction with Jun, I first developed a double-label fluorescence immunocytochemical procedure that permits simultaneous visualization of neurons expressing Fos and Jun. After hypertonic saline administration, the immunostaining of c-Fos and c-Jun was coexistent in the majority of rnagnocellular neurons of SON.

Author(s):
Wang, Ke
Author Organization(s):
Uniformed Services University Of The Health Sciences Bethesda United States
Descriptive Note:
Technical Report
Pagination:
0176

Security Markings

DOCUMENT & CONTEXTUAL SUMMARY

Distribution:
Approved For Public Release
Distribution Statement:
Approved For Public Release;

RECORD

Collection: TR
Subject Terms
Joint Capability Areas:
JCA_5_Command and Control; JCA_5.3_Planning; JCA_1.2.7_Experimentation; JCA_1.2.5_Lessons Learned; JCA_1.2.3_Educating
Modernization Areas:
Biotechnology
Communities of Interest:
Biomedical
Descriptor(s):
proteins, genes, Hybridization, saline solution, deoxyribonucleic acids, Stimulation (Physiology), gene expression, Nervous system, Transcription (Genetics), Hypothalamus, rats, Cytochemistry, immunochemistry, Ribonucleic acids, Plasmids
Keyword(s):
son (supraoptic nucleus), son neurons, DNA Binding, fos, jun, ap 1, dna (deoxyribonucleic acids), Osmotic Stimulation, oncogenes, c fos, c jun, Hypothalamo Neurohypophysial System, Fluid Imbalance, immunocytochemistry, rna (Ribonucleic acids), mrna (micro rna), NORTHERN BLOTTING ANALYSIS
Report Date:
1995 Jun 22
Creation Date:
2017 Feb 02