Correlative Light and Electron Microscopy (CLEM) and its applications in infectious disease

reportActive / Technical Report | Accession Number: AD1009267 | Open PDF

Abstract:

Correlative light and electron microscopy CLEM is an effective technique used to study biological samples. Signal-specific indicators observable under light microscopy LM allow scientists to locate areas of interest for high resolution ultra-structure observation under the electron microscope EM. Recent method developments provided breakthroughs creating an effective, direct, and accurate research diagnostic tool for functionally related structural biological studies. CLEM is particularly useful in infectious disease research where there is need to study, at nanoscale, objects of interest which are commonly part of rare transient events or afflict a particular cell among a majority of unaffected cells. In this review we discuss several CLEM methods, summarize currently available fluorescent markers, and discuss CLEM instrumentation setups for novel approaches to imaging cellular events in infectious disease.

Author(s):
Koistinen, Keith A. ; Blancett, Candace ; Sun, Mei
Author Organization(s):
USAMRIID Frederick United States
Descriptive Note:
Technical Report
Supplementary Note:
Journal of Immunology Research , 01 Jan 0001, 01 Jan 0001,
Pagination:
0023

Security Markings

DOCUMENT & CONTEXTUAL SUMMARY

Distribution:
Approved For Public Release
Distribution Statement:
Approved For Public Release;

RECORD

Collection: TR
Subject Terms
Joint Capability Areas:
JCA_7.2.1_Mitigate Lethal Effects; JCA_7.2_Mitigate; JCA_7_Protection; JCA_6_Net Centric; JCA_3.2_Engagement; JCA_3_Force Application; JCA_6.1.3_Switching and Routing; JCA_6.1_Information Transport; JCA_3.2.2_Non-Kinetic Means; JCA_6.4_Information Assurance; JCA_3.2.1_Kinetic Means; JCA_5_Command and Control
Modernization Areas:
Microelectronics
Communities of Interest:
No COI(s) Identified
Descriptor(s):
ELECTRON MICROSCOPY, electron microscopes, imaging techniques, INFECTIOUS DISEASES
Keyword(s):
clem(Correlative Light Electron Microscopy), biological samples, lm(light microscopy), em(electron microscope)
Report Date:
2016 May 20
Creation Date:
2016 Sep 07