Studies with Fluorescence-Labeled Antibodies. V. Simultaneous Demonstration of Several Antigens by Means of Variously Colored Fluorescent Antibodies, Using Proteus Morganii and Bacillus Cereus as Examples

reportActive / Technical Report | Accession Number: AD0843966 | Open PDF

Abstract:

The labeling of immune gamma globulins with the sulfochlorides of various fluorescent dyes is described. Sulfo-rhodamine B, a pigment with reddish-yellow fluorescence, proved to be a feasible contrasting agent for hydroxy-pyrene-trisulfonic acid chloride and other green-fluorescent labeling substances. P. morganii and Bc. cereus were used to demonstrate that simultaneous specific staining with a mixture of variously labeled antibodies is possible. The potential of the method and its advantages and disadvantages, are discussed.

Author(s):
Poetschke, G. ; Uehleke, H. ; Killisch, L.
Author Organization(s):
ARMY BIOLOGICAL LABS FREDERICK MD
Supplementary Note:
Trans. of Schweizerische Zeitschrift fuer Allgemeine Pathologie und Bakteriologie, v22 p758-765 1959.
Annotation:
Studies with Fluorescence-Labeled Antibodies. V. Simultaneous Demonstration of Several Antigens by Means of Variously Colored Fluorescent Antibodies, Using Proteus Morganii and Bacillus Cereus as Examples Examples--Translation.
Pagination:
0007

Security Markings

DOCUMENT & CONTEXTUAL SUMMARY

Distribution:
Approved For Public Release, Document Partially Illegible
Distribution Statement:
Approved For Public Release; Distribution Is Unlimited. Document Partially Illegible.

RECORD

Collection: TR
Identifying Numbers
Report Number(s):
TRANS-509
Monitor Series:
SMUFD
 Subject Terms
Joint Capability Areas:
JCA_8_Building Partnerships; JCA_8.2_Shape; JCA_3_Force Application; JCA_8.1_Communicate; JCA_3.2_Engagement
Communities of Interest:
Counter WMD
Descriptor(s):
*FLUORESCENT ANTIBODY TECHNIQUES, FLUORESCENCE, TRANSLATIONS, BACILLUS CEREUS, PROTEUS, SWITZERLAND, BIOLOGICAL STAINS, DYES, GAMMA GLOBULIN
Field(s)/Group(s):
Medicine and Medical Research, Microbiology
Keyword(s):
FOREIGN REPORTS
Report Date:
1968 Jul 01