HELPER PHAGE - DEPENDENT TRANSFECTION IN BACILLUS SUBTILIS,

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Abstract:

Bacteriophage SP-18 renders competent cells of Bacillus subtilis 168 ind- susceptible to transfection by DNA from apparently unrelated phages. SP-18 DNA was active in transfection, but SP-10 DNA or LP-19 DNA was inactive when tested alone. However, if SP-10 DNA or LP-19 DNA was added to competent cells along with intact SP-18 phage, infective centers of the corresponding phage, SP-10 or LP-19, were produced. DNA from SP-18 was also active as helper, but it was less effective than the intact phage. Author

Author(s):
Gwinn, Darrel D. ; Thorne, Curtis B.
Author Organization(s):
FORT DETRICK FREDERICK MD
Pagination:
0015

Security Markings

DOCUMENT & CONTEXTUAL SUMMARY

Distribution:
Approved For Public Release

RECORD

Collection: TR
Identifying Numbers
Report Number(s):
SMUFD-Technical manuscript-325
Project Number(s):
DA-1C014501B71A
 Subject Terms
Communities of Interest:
No COI(s) Identified
Descriptor(s):
*BACILLUS SUBTILIS, INFECTIOUS DISEASES, DEOXYRIBONUCLEIC ACIDS, ADSORPTION, BIOASSAY
Field(s)/Group(s):
Microbiology
Keyword(s):
TRANSFECTION
Report Date:
1966 Oct 01