Sorption of Lecithinase 'C' by Ion-Exchange Celluloses,

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Abstract:

The object of the work was to study the sorption of lecithinase C from filtrates of a Clostridium perfrigens culture by anion-exchange celluloses. The lecithinase C was sorbed under dynamic conditions on cellulose ionites from filtrates of the culture, concentrated by precipitation with sodium hexamethaphosphate at a pH of 4.2 in the presence of 15 NaCl. Besides protein components, the concentrate contained substantial quantities of nucleic and pigment impurities. Sorption of lecithinase C from culture filtrates improves with an increase in the exhange capacity of ECTEOLA cellulose and the basicity of the ionite sorption decreases with an increase in NaCl concentration. A simple process was developed for the separation and purification of lecithinase C by successive chromatography on ECTEOLA and DEAE celluloses.

Author(s):
Petrova, L. Ya.
Author Organization(s):
FOREIGN TECHNOLOGY DIV WRIGHT-PATTERSON AFB OHIO
Supplementary Note:
Edited trans. of Nauchno-Issledovatelskii Institut Antibiotikov. Konferentsii Molodykh Uchenykh Leningradskogo Instituta Antibiotikov 2-Y, Leningrad, 4-5 Jun 64. Materialy, n.p., n.d., p64-66, by Dean F. W. Koolbeck.
Annotation:
Sorption of Lecithinase 'C' by Ion-Exchange Celluloses--Translation.
Pagination:
0007

Security Markings

DOCUMENT & CONTEXTUAL SUMMARY

Distribution:
Approved For Public Release

RECORD

Collection: TR
Identifying Numbers
Report Number(s):
FTD-HT-23-977-74
 Subject Terms
Joint Capability Areas:
JCA_8.1.1_Inform Domestic and Foreign Audiences; JCA_8.1_Communicate; JCA_8_Building Partnerships
Communities of Interest:
No COI(s) Identified
Descriptor(s):
*Clostridium perfringens, *Phosphoric monoester hydrolases, *Ion exchange, Sorption, Chromatography, Cellulose, Isolation, Purification, Bacteria, USSR, Translations
Field(s)/Group(s):
Biochemistry, Physical Chemistry
Keyword(s):
*Lecithinases
Report Date:
1974 Apr 18