Purification and Some Chemical and Physical Properties of Staphylococcal Enterotoxin A

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Abstract:

Enterotoxin A from a selected high toxin producing strain 15-25 microgramsml in deep culture of Staphylococcus aureus, designated 13N-2909, has been obtained in highly purified form as indicated by sedimentation velocity, sedimentation equilibrium, disc electrophoresis, and several immunological procedures. Purification was accomplished by the following STEPS 1 removal of the toxin from culture diluted five times with water, and adjusted to pH 5.6 with CG-50 carboxylic acid resin 2 chromatography on carboxymethylcellulose 3 chromatography on hydroxlapatite and 4 filtration on Sephadex G-75. Two major and two minor components were found by isoelectric focusing. The enterotoxin has a molecular weight of 27,800 as determined by sedimentation equilibrium. A value of 27,500 was obtained from polyacrylamide gel electrophoresis in the presence of denaturant. The molecule is a single polypeptide chain with one disulfide bridge and no free sulfhydryl groups. Serine was identified as the C-terminal residue but no free N terminal was found. A complete amino acid analysis is reported.

Author(s):
Schantz, Edward J. ; Roessler, William G. ; Woodburn, Margy J. ; Lynch, Joseph M. ; Jacoby, Henry M.
Author Organization(s):
FORT DETRICK FREDERICK MD
Supplementary Note:
Availability: Pub. in Biochemistry, v11 n3 p360-366 1972.
Annotation:
Reprint: Purification and Some Chemical and Physical Properties of Staphylococcal Enterotoxin A.
Pagination:
0006

Security Markings

DOCUMENT & CONTEXTUAL SUMMARY

Distribution:
Approved For Public Release, Document Partially Illegible
Distribution Statement:
Approved For Public Release; Distribution Is Unlimited. Document Partially Illegible.

RECORD

Collection: TR
Identifying Numbers
Monitor Series:
SMUFD
 Subject Terms
Joint Capability Areas:
JCA_8.1.1_Inform Domestic and Foreign Audiences; JCA_8.1_Communicate; JCA_8_Building Partnerships; JCA_5.3_Planning; JCA_5_Command and Control; JCA_4.5.4_Requirements Definition; JCA_4.5_Operational Contract Support
Modernization Areas:
Directed Energy
Communities of Interest:
No COI(s) Identified
Descriptor(s):
*TOXINS AND ANTITOXINS, *STAPHYLOCOCCUS, CHEMICAL PROPERTIES, PHYSICAL PROPERTIES, CHROMATOGRAPHIC ANALYSIS, STAPHYLOCOCCUS AUREUS, AMINO ACIDS, PROTEINS, PURIFICATION
Field(s)/Group(s):
Biochemistry, Microbiology
Keyword(s):
APATITE
Report Date:
1971 Aug 02