The Nature and Mode of Action of Local Antibody in Intestine

Mouse clostrum IgA were prepared from the first day milk and 2-5 days pooled milk, and IgG and IgM were prepared from adult mice sera which were obtained from mice immunized with soluble cell-free cholera vaccine. Rabbit antisera against those immunoglobulins were prepared. Anti-IgA prepared from the first day milk was better than that of prepared from the 2-5 days pooled milk. The first day milk was rich in IgA and 2-5 days milk was rich in IgM. Almost all of feces of mice which were immunized with cell-free cholera vaccine contained IgA while very small number of feces from non-immunized mice contained IgA. IgM was not detected in feces but was detected in some of the extract of intestinal tissues from immunized mice. No IgG was detected in feces or in extracts of intestinal tissues regardless of whether the mice was immunized or not.