Recovery of White Blood Cells After Freezing.

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Abstract:

Whereas the red cells recover from the actual congelation of all the water freezable at -3C, the neutrophils are already injured when only a fraction of the water freezable at -1.5C is congealed. Electron microscope studies of freeze-dried or freeze-subsituted suspensions of leucocytes indicated that freezing at rates varying from 30 to 2000 degrees C per second results always in the formation of intracellular and intranuclear ice in neutrophils. A study by concentrations of solutions of the cryoprotectants ethylene glycol, glycerol and polyvinyl pyrrolidone, undergo homogeneous nucleation throws some light on the conditions under which ice nuclei may originate in cryoprotected biological material. Author

Author(s):
Luyet, Basile J. ; Menz, Leo J. ; Rapatz, Gabriel L. ; Rasmussen, Don.
Author Organization(s):
AMERICAN FOUNDATION FOR BIOLOGICAL RESEARCH MADISON WIS
Descriptive Note:
Final rept.,
Pagination:
0020

Security Markings

DOCUMENT & CONTEXTUAL SUMMARY

Distribution:
Approved For Public Release

RECORD

Collection: TR
Identifying Numbers
Report Number(s):
ONR-3700
Contract/Grant Number(s):
Nonr-3700(00)
Project Number(s):
NR-105-267
Monitor Series:
3700
 Subject Terms
Communities of Interest:
No COI(s) Identified
Descriptor(s):
(*LEUKOCYTES, FREEZING), (*BLOOD CELLS, PRESERVATION), ELECTRON MICROSCOPY, VIABILITY
Field(s)/Group(s):
Anatomy and Physiology
Keyword(s):
NEUTROPHILS, *BLOOD PRESERVATION, CRYOBIOLOGY
Report Date:
1971 Jul 27