A RAPID METHOD OF OBTAINING ANTIBODIES LABELLED WITH FLUORESCENT STAINS

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Abstract:

This report describes a new method for the isolation of immune euglobulins antibodies from agglutinating anti-plague and anti-cholera serums and a new method of purifying the labelled antibodies from the fluorochrome excess. With the new method of isolating and purifying antibodies, the time required to prepare luminescent conjugates is shortened from 3-4 weeks to 3-4 days. The suggested method of preparing fluorochrome-labelled antibodies is realizable in any laboratory, since it requires no special equipment refrigeration centrifuges and rooms. The advantages of a new luminescent stain -- fluorescein isothiocyanate, over fluorescein isocyanate are shown. The immune euglobulins isolated by the new method were classifiable among the gamma- globulins in their electrophoretic composition.

Author(s):
Tabakov, P. K. ; Chibrikova, E. V. ; Shurkina, I. I. ; Velner, E. I.
Author Organization(s):
ARMY BIOLOGICAL LABS FREDERICK MD
Supplementary Note:
Trans. of Zhurnal Mikrobiologii, Epidemiologii i Immunobiologii (USSR) v33 n10 p26-30 1962.
Annotation:
A rapid method of obtaining antibodies labelled with fluorescent stains--Translation
Pagination:
0009

Security Markings

DOCUMENT & CONTEXTUAL SUMMARY

Distribution:
Approved For Public Release, Document Partially Illegible
Distribution Statement:
Approved For Public Release; Distribution Is Unlimited. Document Partially Illegible.

RECORD

Collection: TR
Identifying Numbers
Report Number(s):
TRANS-1032
Monitor Series:
SMUFD
 Subject Terms
Communities of Interest:
Air Platforms
Descriptor(s):
*FLUORESCENT ANTIBODY TECHNIQUES, USSR, FLUORESCENCE, PURIFICATION, ANTIBODIES, TRANSLATIONS, DYES, IMMUNE SERUMS, ANTIGENS, ELECTROPHORESIS, SERODIAGNOSIS, ANTIGEN ANTIBODY REACTIONS, GAMMA GLOBULIN
Field(s)/Group(s):
Biochemistry, Microbiology
Keyword(s):
FOREIGN REPORTS
Report Date:
1968 Jul 01