FLUORESCENT CELL-COUNTING ASSAY OF YELLOW FEVER VIRUS

Hahon, Nicholas

FLUORESCENT CELL-COUNTING ASSAY OF YELLOW FEVER VIRUS

Active / Technical Report | Accession Number: AD0469383 |

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Abstract:

A quantitative assay of infective yellow fever virus particles was developed that is based on the enumeration of cells containing fluorescent viral antigen 24 hours after infection of coverslip McCoy cell cultures. The rapidity and efficiency of virus adsorption onto cell monolayers was markedly enhanced by centrifugation of virus inoculum. By this procedure, 95 of virus was adsorbed within 15 minutes and a proportionality was demonstrated between the number of fluorescent cells and the volume of inoculum. The incubation period of 24 hours was established from observations on the sequential development of viral antigen within cells and fluorescent cell counts. The relationship between virus concentration and cell-infecting units of virus was linear the distribution of fluorescent cells in cell monolayers was random. The sensitivity of the fluorescent cell-counting assay was comparable to that of the method of intracerebral inoculation of mice. A quantitative linear relationship was demonstrated between the quantity of virus neutralized and dilutions of antiserum.

Author(s):

Hahon, Nicholas

Author Organization(s):

ARMY BIOLOGICAL LABS FREDERICK MD

Descriptive Note:

Technical Manuscript

Supplementary Note:

DOI: 10.21236/AD0469383

Pagination:

0022

Security Markings

DOCUMENT & CONTEXTUAL SUMMARY

Distribution:

Approved For Public Release, Document Partially Illegible

Distribution Statement:

Availability: Document Partially Illegible.

RECORD

Collection: TR

Identifying Numbers

Report Number(s):

ABL-TM-235

Project Number(s):

1C522301A084

Monitor Series:

ABL/MD

Subject Terms

Joint Capability Areas:

JCA_6_Net Centric; JCA_3.2_Engagement; JCA_3_Force Application; JCA_3.2.2_Non-Kinetic Means; JCA_6.4_Information Assurance; JCA_8.1.1_Inform Domestic and Foreign Audiences; JCA_8.1_Communicate; JCA_8_Building Partnerships

Communities of Interest:

Air Platforms

Descriptor(s):

*BIOASSAY, *YELLOW FEVER VIRUS, DETECTION, FLUORESCENCE, ADSORPTION, COUNTING METHODS, SENSITIVITY, CELLS(BIOLOGY), CULTURES(BIOLOGY), LABELED SUBSTANCES, BIOLOGICAL STAINS

Field(s)/Group(s):

Microbiology, Pharmacology

Report Date:

1965 Jul 01