ATTENUATED TOTAL REFLECTANCE IN INFRARED SPECTROPHOTOMETRY OF BIOLOGICAL SYSTEMS
Abstract:
The attenuated total reflectance ATR method for obtaining infrared absorption spectra has been shown to be a feasible technique for the study of biological and other materials in their normal aqueous environment. Water-compensated ATR spectra for a number of simple compounds, a protein bovine serum albumin, and a bacterium Serratia marcescens in water solution or suspension, were generally similar to normal transmission spectra for dried samples but showed some distinctive features not present in the latter. Some limitations of the method and steps taken to overcome them are discussed.
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Collection: TR