ATTENUATED TOTAL REFLECTANCE IN INFRARED SPECTROPHOTOMETRY OF BIOLOGICAL SYSTEMS

reportActive / Technical Report | Accession Number: AD0459388 | Open PDF

Abstract:

The attenuated total reflectance ATR method for obtaining infrared absorption spectra has been shown to be a feasible technique for the study of biological and other materials in their normal aqueous environment. Water-compensated ATR spectra for a number of simple compounds, a protein bovine serum albumin, and a bacterium Serratia marcescens in water solution or suspension, were generally similar to normal transmission spectra for dried samples but showed some distinctive features not present in the latter. Some limitations of the method and steps taken to overcome them are discussed.

Security Markings

DOCUMENT & CONTEXTUAL SUMMARY

Distribution:
Approved For Public Release
Distribution Statement:
Approved For Public Release; Distribution Is Unlimited.

RECORD

Collection: TR
Identifying Numbers
Subject Terms