MECHANISM OF ENZYME ACTION.

The carboxylic groups and SH groups play impor tant roles in hydrolytic plant-enzymes like papain and ficin. A cooperative effect of imidazole groups and mercapto or carboxylic groups are important in the action of several glycolytic enzymes. In addition, electrostatic attraction to the carboxylate anion in choline esterase may help the catalytic hydrolysis of the imidazole groups on the enzyme from a neighboring group position. The synthetics of homopolymers, copolymers and appropriate substrates and the kinetic studies performed so far were done in order to attain further insight into the mechanism of action of the afore mentioned hydrolytic enzymes. Author