THE EXPLANATION OF PATHOGENESIS OF JAPANESE ENCEPHALITIS (JE) VIRUS AND THE ESTABLISHMENT OF AN ATTENUATED STRAIN OF JE VIRUS COMPLETELY A VIRULENT TO MAN. PART III. STUDIES ON JAPANESE ENCEPHALITIS VIRUS INFECTION WITHIN CULTIVATED ANIMAL CELLS BY THE APPLICATION OF FLUORESCENT ANTIBODY TECHNIQUE.

Process of Japanese B encephalitis virus infection cultivated animal cells. i.e. hamster kidney, porcine kidney and HeLa cells was fol lowed by fluorescent antibody technique. The infection was adjusted to obtain multiplicities 5-10 for each system Viral antigen was initial ly detected in perinuclear cytoplasm at 10 - 15 hrs of infection. then numbers of the antigen producing cells increased and antigen producing area expanded within cytoplasm.