Unpredictable aggressive behavior by youth with autism spectrum disorder (ASD) isolates them from educational, social and family activities. Approximately 2/3 of youth with ASD display aggression, a common reason for treatment referral; yet evidence-based pharmacological and behavioral interventions for aggression in ASD are frequently ineffective. Aggression is particularly impairing in the 30-40% of youth with ASD who are minimally verbal (MV-ASD). Aggression may represent a maladaptive attempt to express or modulate physiological arousal arising from distress. We hypothesize that physiological arousal precedes aggressive behavior. We aim to predict aggression in MV-ASD before it occurs using data collected from wrist-worn physiological sensors and behavior observation. Using sophisticated machine learning algorithms linking observable aggression to preceding physiological signals (heart rate, skin conductance), we may identify new opportunities for intervention. Since project launch, we refined data collection procedures, established processes for behavioral data upload and physiological data transfer to collaborators at NEU, and implemented physiological data quality checks. Staff training was completed on all procedures including use of biosensors and a smartphone application to code aggression instances, at a high level of inter-rater reliability. 49 youth were enrolled and data collection was completed with 24. Behavioral data was transferred to collaborators at NEU, physiologic data was made available to NEU collaborators via the cloud, and phenotypic data was transferred to NEU collaborators via RED cap.