DID YOU KNOW? DTIC has over 3.5 million final reports on DoD funded research, development, test, and evaluation activities available to our registered users. Click
HERE to register or log in.
Accession Number:
AD1173716
Title:
Analysis of Generation and Sampling Methods for MS2 Virus Aerosols
Report Date:
2022-07-01
Abstract:
This report provides methodology and results from experiments conducted to characterize generation and sampling methods for enterobacteria phage Emesvirus zinderi (MS2) viral aerosols. Three aspects were evaluated: (1) the aerosol generation methods, (2) the sampling and analysis methods, and (3) the environmental conditions and growth media on the viability of a viral aerosol. Different methods for aerosol generation were also evaluated, including the Collison nebulizer, bubble generator, noncirculating bubbler, spinning-top aerosol generator, and ultrasonic nebulizer. Various methods exist for sampling and analyzing viral aerosols, which have specific requirements because of their size and biological properties. Bioaerosol sampling methods assessed include filter collection, impingement into fluid, and impaction onto agar. Viral aerosol quantification methods that were evaluated include an ultraviolet aerodynamic particle sizer and an electrical low-pressure impactor. Viral viability can be affected by temperature, humidity, and media. Viability was evaluated within a 20 to 50 C temperature range and at 30 and 65 humidity. Five sampling media were used: phosphate-buffered saline, tryptone (15 g/L), glycerol (0.1 ), skim milk (3 g/L), and albumin (1 g/L).
Document Type:
Conference:
Journal:
Pages:
52
File Size:
5.31MB
Contracts:
Grants:
Distribution Statement:
Approved For Public Release
