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Controlled Release of Inactivated Chikungunya Virus Vaccine Candidate: A Single Vaccination Approach for Long-Term Immunity


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Requirement of multi dose regimen of vaccines presents a barrier to protect from vaccine preventable disease. Problem is amplified in case of emerging infectious viruses where either there is a lack of live attenuated strain of virus to immunize and for inactivated vaccine not much time is at hand to perform multiple immunizations. Chikungunya virus (CHIKV) has caused large outbreak in tropical countries across the globe. There is no FDA approved vaccine against CHIKV. In this study gamma-radiation inactivated CHIK181/25 is encapsulated in lipid nanoparticles (HALNP) which will be embedded in the polyelectrolyte multilayer (PEM) films for temporal release in vivo. This platform will provide a single-shot vaccine of inactivated-CHIKV181/25 for intermittent release of antigen mimicking primary and booster regimen of multi-dose vaccination.CHIKV181/25 was inactivated by gamma radiation using a unique approach of protecting viral proteins while allowing for homogenous inactivation of the virus. Protection of epitopes on inactivated-CHIKV181/25 was determined and encapsulation of inactivated-CHIKV181/25 in HALNP was performed. Mice were immunized with HALNP encapsulated inactivated-CHIKV181/25 and anti-CHIKV antibody titers were evaluated to establish antigen preservation post encapsulation. Experiments are now planned to test the long-term immunity following immunization with HALNP-encapsulated inactivated CHIKV181/25 embedded in the PEM lattice. This study addresses an FY17 PRMRP topic "Vaccine Development for Infectious Disease" and Area of Encouragement under "Development of vaccines to prevent U.S. Service members from becoming ill from endemic disease exposure during operational deployments including arthropod-borne diseases such as chikungunya virus"



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