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Understanding the 'Mission Versatility' of Membrane Proteins via Nanoscopic Imaging
The goal of this AFOSR Young Investigator Program grant is to investigate the nanoscale structural and functional dynamics of membrane proteins in their native liquid environment using liquid phase transmission electron microscopy (TEM). Over the project time period, we optimized the self-assembly and purification of nanodiscsmembrane proteins in a single-particle form amenable to TEMobservationcontaining the protein cytochrome p450 2J2 (CYP-2J2) and then developed a graphene liquid sandwich to encapsulate CYP- 2J2 nanodiscs in their native buffer conditions for imaging.
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