The goal of this research is to evaluate the efficacy of our new surgical technique called nerve-muscle-endplate band grafting (NMEG) for limb reinnervation in a rat model. Immediate transection of the nerve innervating the tibialis anterior (TA) muscle, a NMEG pedicle was harvested from the ipsilateral lateral belly of the gastrocnemius muscle (GM) and implanted to the native motor zone (NMZ) of the TA muscle for reinnervation. Three months after surgery, functional recovery of the TA treated with the NMEG-NMZ technique was evaluated by muscle force measurement. The extent of axonal regeneration in the target muscle was assessed using immunohistochemical techniques. Our results showed that NMEG-NMZ technique resulted in better functional recovery (81 of the control) as compared with commonly used nerve end-to-end anastomosis (EEA) (50 ). The NMEG-NMZ induced extensive axonal regeneration in the treated muscle. These findings suggest that NMEG-NMZ technique is effective for limb reinnervation.