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Accession Number:
AD1135306
Title:
Gut and Cartilage Microbiomes as Novel Mediators of Inflammation in and Potential Therapy for Post-Traumatic Osteoarthritis
Report Date:
2021-02-01
Abstract:
In this study we plan to evaluate both the pathogenic and potential protective effects of changes in body microbiomes along with their interactions with the immune system in PTOA, using cutting-edge microbiome and immune cell subtyping analyses. In Aim 1 (year 1), we will evaluate correlations between gut and cartilage microbiomes in PTOA-susceptible C57BL/6J (B6) and PTOA-resistant MRL/MpJ mice (MRL) before and after DMM-induced PTOA, and correlate these findings with systemic inflammatory cell responses via peripheral blood cell phenotyping. In Aim 2 (year 2), we will establish the direct contributions of the gut microbiome in both establishment of a cartilage microbiome and in PTOA pathogenesis by introducing the gut microbiota of B6 and MRL mice into germ-free (GF) mice, followed by DMM. In Aim 3 (year 2), we will examine the potential therapeutic potential of altering the gut microbiota as a preventative / therapeutic for PTOA by conducting gut microbiome transplantation of superhealer MRL into non-healer B6 mice, measuring susceptibility to OA, and quantifying associated systemic inflammatory cell changes.
Document Type:
Conference:
Journal:
Pages:
15
File Size:
0.48MB
W81XWH-20-1-0002
(W81XWH2010002);
Contracts:
Grants:
Distribution Statement:
Approved For Public Release
