View The Document

Accession Number:

AD1058598

Title:

Improving Protein Stability and Activity Using Zwitterionic Peptides

Author(s):

Author Organization(s):

Report Date:

2017-03-13

Abstract:

This work established the use of polypeptides composed of repeating glutamic acid (E) and lysine (K) (poly(EK)) for the purpose of increasing protein stability. The addition of poly(EK) to the primary sequence at the C-terminus of a model beta-lactamase protein and a destabilized TEM-19 demonstrated significant ability in increasing protein stability while maintaining bioactivity. The use of fusion protein products allows for a one-step protocol resulting in a homogeneous product, a significant advantage over polymer-based approaches.

Pages:

11

File Size:

0.46MB

Descriptors:

Identifiers:

SubjectCategory:

Distribution Statement:

Approved For Public Release

View The Document