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Accession Number:
ADB104013
Title:
Togavirus-Specific Immune Effector Mechanisms.
Corporate Author:
MARYLAND UNIV BALTIMORE SCHOOL OF MEDICINE
Report Date:
1983-12-01
Abstract:
Interactions between alphaviruses, antibodies, and cells were examined as part of an overall effort to characterize relevant antigens and immune effector mechanisms. Antibody-dependent complementC-dependent cytolysis was sed to demonstrate that alphaviruses induce serum antibodies capable of lysing cells infected with heterologous alphavirus species. Similar ross-reactive antibodies were sought, with partial success, in our existing battery of monoclonal MC antibodies prepared against the Sindbis virus SIN envelope glycoproteins, E1 and E2. Only two MC antibodies were found to react not only with SIN and western equine encephalitis virus WEE but with Venezuelan equine encephalitis virus VEE and Semliki Forest virus SF these El-specific MC antibodies mediated C-dependent lysis of VEE-infected cells and one protected mice against lethal VEE challenge. Neither lysed SF-infected cells and the one tested in vivo did not protect against SF challenge. MC antibodies were used to determine topographical relationships among viral epitopes bound by protective MC antibodies. A sandwich antigen-captue enzyme-linked immunosorbent assay ELISA was used to detect nanogram quantities of SIN antigen and to distinguish between epitopes on virion surfaces and those that became inaccessible to antibodies during viral morphogenesis at the cell surface.
Descriptive Note:
Annual progress rept. 1 Jan-31 Dec 83,
Pages:
0056
Distribution Statement:
Approved for public release, distribution unlimited.
Contract Number:
DAMD17-81-C-1027
Contract Number 2:
DAMD17-78-C-8042
File Size:
0.00MB
