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Accession Number:
ADA067584
Title:
Development of an On-Line Biological Detector.
Corporate Author:
GULF SOUTH RESEARCH INST NEW ORLEANS LA
Report Date:
1977-10-30
Abstract:
Artificial capillary perfusion culture of mammalian cells is being tested for its effectivenes as a detector of toxic substances. Using change in cellular oxygen utilization rate as a measure of toxicity, the effects on L929 cells of o-toluidine, hydroquinone, 2,3-dimethylphenol, 2,4-dinitrophenylhydrazine, potassium cyanide, N,N-diethyl-m-toluamide and sodium iodoacetate have been studied. The toxic substance is added to the medium stream, and after 60-90 minutes exposure of the cells, curves of log dose versus percent inhibition of oxygen utilization are comparable to those obtained for log dose versus percent inhibition of cell growth. Adaptation of the perfusion system for water toxicity screening and for use outside a warm room was explored. A prototype detector flow system, which utilizes once-through perfusion, is described. Author
Descriptive Note:
Final rept. 31 Jun 75-31 May 77,
Pages:
0047
Contract Number:
DAMD17-75-C-5075
File Size:
18.54MB
