An Oil Spill Surveillance System based on remote measurement of petroleum oil fluorescence was designed, built, and tested. This system is capable of oil spill detection and oil classification in a commercial harbor environment at a range in excess of 100 meters during hours of darkness. The system functions at viewing angles varying from vertical to within 10 degrees of the horizon and may be programmed for area detection scanning. Fluorescence spectra of a range of oils are presented including corrected spectra presented in a two-dimensional format. General classification of oil type through fluorescence measurements is discussed. A method for the estimation of film thickness from remote fluorescence measurement is described and a polarization technique for discriminating between marine and oil fluorescence is presented.