The objective of this project is to elucidate the immunological mechanisms induced by live-attenuated and purified inactivated dengue virus vaccines administered in a heterologous prime-boost regimen. Innate and adaptive T and B cell responses will be measured using molecular and cellular approaches and the data analyzed using a systems biology approach. During the third project year, we performed genomic characterization of study subjects using molecular HLA typing, RNA-seq of unfractionated PBMC, and single-cell RNA-seq of PBMC. Genomic data were analyzed for associations with vaccine regimen and measures of vaccine immunogenicity. Preliminary findings from these analyses are being explored further.