Vanderbilt University Medical Center Nashville United States
The objective of these studies is to determine the role of human herpesviruses and antiherpesvirus immunity in the development of idiopathic pulmonary fibrosis. The first aim of the study is to collect peripheral blood and broncholalveolar lavage fluid from patients with IPF or other ILDs undergoing clinical bronchoscopy, quantify viral load and perform immunophenotyping of circulating and BAL immune cells. To date, enrollment is continuing and we are proceeding with characterization of immune cell populations as planned. The second aim is to use a model of genetic risk for IPF to investigate the mechanisms of herpesvirus-driven experimental fibrosis. Our first objective was to determine the T-cell profiles during the time course of MHV68 infection. We found that there is expansion of the T-cell compartment along with upregulation of T-cell exhaustion markers that peaks 14-28 days after infection. We plan to continue studies as outlined in the SOW for the next funding period.