The University of Texas MD Anderson Cancer Center Houston United States
Our IDEA is on the right track. We have successfully inserted a fluorescent marker mOrange into MITs Dr. Zhangs pLenti-Crispr-v2, making transfection into mammalian cells easier and visible under fluorescent microscope, it the same time, those cells under Crispr editing are also selectable with puromycin. We have successfully knocked-out RhoA expression in cell lines of AGS and GT5.With RhoA knockout in cell line GT5, Yap1 is also significantly downregulated. Reintroducing mutant RhoA-Y42C is in progress.