Accession Number:

AD1046138

Title:

Exploiting RhoA Mutations in Diffuse Gastric Adenocarcinoma and Targeting Intertwined RhoA and Yap1 Pathways for Therapeutic Advantage

Personal Author(s):

Corporate Author:

The University of Texas MD Anderson Cancer Center Houston United States

Report Date:

2017-10-01

Abstract:

Our IDEA is on the right track. We have successfully inserted a fluorescent marker mOrange into MITs Dr. Zhangs pLenti-Crispr-v2, making transfection into mammalian cells easier and visible under fluorescent microscope, it the same time, those cells under Crispr editing are also selectable with puromycin. We have successfully knocked-out RhoA expression in cell lines of AGS and GT5.With RhoA knockout in cell line GT5, Yap1 is also significantly downregulated. Reintroducing mutant RhoA-Y42C is in progress.

Descriptive Note:

Technical Report,15 Sep 2016,14 Sep 2017

Pages:

0034

Descriptors:

Identifiers:

Subject Categories:

Distribution Statement:

Approved For Public Release;

File Size:

1.59MB

View The Document