University of Mississippi Medical Center Jackson United States
The major goal of this application is to determine whether prostate cancer cells express enhancer RNAs in response to androgen treatment such that these enhancer RNAs may serve as novel biomarkers for prostate cancer diagnosis and prognosis. There are two Tasks in this application. First, we will perform global run-on assay and deep sequencing to identify AR-specific enhancer RNAs. Second, we will validate them and then determine their functional significance. The success of this study may lead to novel clinical applications such as identification of biomarkers or therapeutic targets for prostate cancer, especially for castration resistant prostate cancer.