MASSACHUSETTS GENERAL HOSPITAL BOSTON United States
The overall goal of this project is to explore a new, chip-based magnetic sensor that will allow for fast and sensitive molecular analyses of tumor cells. To achieve this goal, we focused on i implementing a miniaturized Hall Hall sensor system and ii synthesizing magnetic nanoparticles for cell labeling. Sensor system. The Hall sensor has a hybrid structure, consisting of an Hall integrated-circuit IC chip with a microfluidics on top. The IC chip contains an array of micrometer-scale Hall elements for magnetic detection. To facilitate the system control, we also integrated control electronics in the same chip. By fabricating such ICs through a semiconductor foundry, we could produce a large number of chips approximately 120 ICs with tight quality control. Magnetic nanoparticles MNPs. We have synthesized a panel of MNPs with varying diameters 10, 12, 16 nm each particle type displayed a unique magnetic property, which will be exploited for multiplexed detection. To make particles water-soluble and amenable for cell targeting, we further coated them with biocompatible polymers. We now plan to apply the developed platforms to detect ovarian cancer cells spiked in the whole blood.