Trustees of the University of Pennsylvania Philadelphia United States
The role of tumor-associated neutrophils TANs in cancer progression remains unclear and has only been recently investigated in murine tumor models. However, there are limited data about the TANs in human tumor. The goal of this study is to provide a phenotypic and functional characterization of TANs in lung cancer patients. We have identified two major subsets of TANs in lung tumors canonical TANs that express classic neutrophil markers and APC-like hybrid TANs that display a combination of canonical neutrophil markers and markers of antigen-presenting cells. In this study we have performed analysis of key inflammatory factors secreted by canonical and hybrid neutrophils. We have also performed whole human genome RNA expression profiles of these neutrophil subsets. We found that the APC-like hybrid neutrophils are superior to canonical neutrophils in their ability to trigger and support T cell responses in direct cell-cell interactions.