NORTH CAROLINA STATE UNIV AT RALEIGH RALEIGH United States
A recently developed class of models incorporating the cyton model of population generation structure into a conservation-based model of intracellular label dynamics is reviewed. Statistical aspects of the data collection process are quantified and incorporated into a parameter estimation scheme. This scheme is then applied to experimental data for PHA-stimulated CD4 T and CD8 T cells collected from two healthy donors. This novel mathematical and statistical framework is shown to form the basis for accurate, meaningful analysis of cellular behaviour for a population of cells labeled with the dye carboxyfluorescein succinimidyl ester and stimulated to divide.
Journal of Biological Dynamics , 7, 1, 01 Jan 0001, 01 Jan 0001, Open Access: Publishers Version. May be placed on public websites; Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.