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Accession Number:
AD0872692
Title:
An Improved Heat-Stable Glutamine-Free Chemically Defined Medium for Growth of Mammalian Cells
Corporate Author:
FORT DETRICK FREDERICK MD
Report Date:
1970-08-01
Abstract:
A heat-stable chemically defined medium, free of glutamine, is described for the growth of mammalian cells in suspension culture. The presence of L-alanine in the defined medium permitted the omission of glutamine. A 22- fold increase in the population of a substrain of mouse L cells was obtained in 6 days with no medium replenishment during incubation. Maximum yields were obtained by daily medium replacement and venting of cultures. Growth was also improved in a line of cat kidney cells and HeLa cells, and in another substrain of L cells.
Pages:
0013
Distribution Statement:
Approved for public release; distribution is unlimited. Document partially illegible.
File Size:
0.44MB
