DID YOU KNOW? DTIC has over 3.5 million final reports on DoD funded research, development, test, and evaluation activities available to our registered users. Click HERE
to register or log in.
Replication of Venezuelan Equine Encephalomyelitis Virus in Vitro: I. Growth in Suspension Cell Cultures Grown in Serum-Free and Defined Media
FORT DETRICK FREDERICK MD
Various mammalian cells propagated in serum-free and chemically defined media yielded high titers of Venezuelan equine encephalomyelitis VEE virus. Some difference in maximal titers was noted, depending upon the medium employed. Of the two serum-free media tested, lactalbumin hydrolysate medium was more effective than the chemically defined medium in stimulating viral growth. The addition of serum to serum-free cultures at the time of viral inoculation had a pronounced effect characterized first by a delay and then by a burst of viral replication to very high titers. Thus, the levels of Vee virus replication appear to be influenced by a variety of nutritional factors whose mechanisms of action have yet to be elucidated.
Approved for public release; distribution is unlimited. Document partially illegible.