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Accession Number:
AD0871977
Title:
Replication of Venezuelan Equine Encephalomyelitis Virus in Vitro: I. Growth in Suspension Cell Cultures Grown in Serum-Free and Defined Media
Corporate Author:
FORT DETRICK FREDERICK MD
Report Date:
1970-06-01
Abstract:
Various mammalian cells propagated in serum-free and chemically defined media yielded high titers of Venezuelan equine encephalomyelitis VEE virus. Some difference in maximal titers was noted, depending upon the medium employed. Of the two serum-free media tested, lactalbumin hydrolysate medium was more effective than the chemically defined medium in stimulating viral growth. The addition of serum to serum-free cultures at the time of viral inoculation had a pronounced effect characterized first by a delay and then by a burst of viral replication to very high titers. Thus, the levels of Vee virus replication appear to be influenced by a variety of nutritional factors whose mechanisms of action have yet to be elucidated.
Supplementary Note:
DOI: 10.21236/AD0871977
Pages:
0020
Distribution Statement:
Approved for public release; distribution is unlimited. Document partially illegible.
File Size:
0.69MB
