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Accession Number:
AD0857268
Title:
A Plaque Assay System for Several Species of Rickettsia,
Corporate Author:
FORT DETRICK FREDERICK MD
Report Date:
1969-06-01
Abstract:
The plaque assay procedure developed in this laboratory for the Bitter Root strain of Rickettsia rickettsii has recently been shown to be appropriate for other rickettsiae. Large 2 mm distinct plaques were formed when chick primary monolayers were infected with R. rickettsii Sheila Smith strain, R. akari MK strain, R. conorii, R. siberica, or R. australis. Somewhat smaller plaques were formed by R. mooseri Wilmington strain and the Breinl and Madrid E strains of R. prowazekii. A comparison of the plaque titers and the yolk sac 50 lethal dose for each species showed that the plaque assay was more sensitive than the yolk sac titration procedure. The plaque morphology of rickettsiae of the spotted fever group was similar plaques produced by the typhus group were also basically similar, suggesting that plaque morphology may become important as a diagnostic tool for studying rickettsiae. Author
Pages:
0013
File Size:
0.00MB
