In this investigation, observations were carried out on the immunological behavior in vivo and in vitro of the polysaccharidic fraction isolated by the Fuller technique, starting with Br. abortus B99. It was found that the fraction being investigated was a complex hapten, atoxic to rabbits and sorologically active in precipitation tests. In animals that had been previously innoculated with Brucella, the injection of the polysaccharidic antigen did not induce reactions of allergic nature, although it did give a passive cutaneous anaphylaxis test. At the same time, its innoculation did not produce any change of temperature, weight, leucocytic formula, or electrophoretic profile of the sera of the animals.
Trans. of Universidade do Brasil, Rio de Janeiro. Laboratorio de Microbiologia. Anais de Microbiologia, n10 p79-90, 1962.