The hemagglutinin of the yellow-fever virus can be established quantitatively in infected human explants both in the tissue through alkaline extraction with borate buffer and in the liquid culture phase after elimination of nonspecific inhibitors with freon. Hemagglutinin can be established in the explanted tissue already before the cytopathogenic effect and it reaches its highest concentration at the time of the maximum infectiousness titer. The release of hemagglutinin into the culture medium takes place in a synchronized fashion with the progression of the cytopathogenic effect. There is a certain porportionality between the hemagglutinin and the infectiousness titer.
Trans. of Archiv fuer Virusforschung (Switzerland) n10 p267-287 1961.