DID YOU KNOW? DTIC has over 3.5 million final reports on DoD funded research, development, test, and evaluation activities available to our registered users. Click
HERE to register or log in.
Accession Number:
AD0838752
Title:
A METHOD FOR PREPARING SMALL QUANTITIES OF C14-LABELED STAPHYLOCOCCAL ENTEROTOXIN B
Corporate Author:
FORT DETRICK FREDERICK MD
Report Date:
1968-06-01
Abstract:
A procedure is described for production from Staphylococcus aureus of small amounts of radioisotopically labeled staphylococcal enterotoxin B for animal metabolism studies. The procedure is a modified scaled-down adaptation of the Schantz method of enterotoxin production. The organism is cultured on medium containing C 14-labeled yeast protein hydrolyzate, and the toxin is purified by adsorption on and elution from Amberlite CG-50 resin columns. The procedure is capable of yielding 12 to 16 mg of C 14-labeled enterotoxin B of high purity 96 to 100 with an isotope concentration of 0.2 microcurie per mg.
Supplementary Note:
DOI: 10.21236/AD0838752
Pages:
0010
Distribution Statement:
Approved for public release; distribution is unlimited., Availability: Document partially illegible.
File Size:
0.42MB
