A method for the study of plant tissue with the aid of fluorescent antibodies is described. Tobacco plants are examined at known intervals after infection with tobacco mosaic virus. Viral protein is demonstrable already 45 hours after infection. It appears first in the cytoplasm, in a perinuclear zone, and spreads subsequently over the entire cytoplasm. No virus protein is seen in the nuclei and chloroplasts. Synthesis of viral protein consequently seems to occur exclusively in the cytoplasm. This fact is supported also by fractionation of infected tissue. Here the fluorescent antibody reveals viral antigen principally in the microsomal fraction.
Trans. of Zeitschrift fuer Naturforschung (West Germany) v14b p510-515 1959.