It was confirmed histo-serologically with the aid of liver-specific fluorescein-labeled antibodies that the liver-specific antigen cannot be demonstrated in primary rat liver cell carcinoma. The liver-specific antigen is localized exclusively in the parenchymal cells of the liver. Liver tissue exposed to the carcinogen contains less liver-specific antigen than the normal liver. This is expressed in the complement fixation test by a reduced liver- specific serologic activity of the microsomes and mitochondria, and histo- serologically in the appearance of characteristic tissue islands in which the antigenic concentration is decreased in comparison to the neighboring tissue. The degree of antigenic loss depends upon the total dose of carcinogen administered and on the latent period.
Trans. of Zeitschrift fuer Naturforschung (West Germany), v11b, p31-38, 1956.