A quantitative method for the analysis of bacterial mixtures, especially those of the stool was applied to the analysis of infant feces. The principle of the method involves cultivation of the various bacterial species or strains from the stools, production of antisera and their linkage to a fluorescent dye. The labeled antisera are used in differential staining of stool smears. The percentage of specifically stained bacteria on the smear is determined by observation or photography under ultraviolet light, followed by visualization of the same field of view in visible light under dark field illumination. The absolute content of specifically stained organisms in the stool and their growth rate during culture may be established by determining the absolute bacterial count in a chamber and by quantitative studies of cultures. Thus, the method offers information on the value of germ counts in selective media.
Trans. of Schweizerische Zeitschrift fuer Allgemeine Pathologie und Bakteriologie, v22 p747-751, 1959.