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STUDIES ON THE CULTURE OF BACTERIUM ORYZAE (UYEDA ET ISHIYAMA) NAKATA
FORT DETRICK FREDERICK MD
Growth of this bacterium was best on bouillon glucose agar and potato sucrose agar, with the growth being relatively poor in the cases of the other media tested. Between the sugars sucrose, dextrose, and maltose, growth was equally good, and the degree of growth was fairly uniform for the range of sugar concentration 0.5 to 10.0. The saccharinity of the bouillon was higher than that of the potato broth. In the case of the sugar bouillon, it was seen that the meat extract, the peptone, as well as the sugar were all essential to bacterial growth, and also that there was little difference between the use of bonito or beef meat extracts. It was found that growth occurs for pH values in the range 4.0-8.8 only. Comparing the slant culture specimens with those of the stab culture, it was observed that in the early stages, growth seemed to be more favorable with the stab culture specimens. This could be due to a denser bacteria population transplanted by the stab method of inoculation, or to some secretion of the multiplying bacteria which makes the nutrients more easily absorbable.
Trans. of Nihon Shokubutsu Byori Gakkaiho (Japan) v17 n2 p73-5 1953.
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