Blood collected for transfusion purposes is usually stored in acid-citrate-dextrose ACD or citrate-phosphate-dextrose CPD at 4C. These preservatives maintain red cell ATP concentrations and thus viability of the cells during storage. Since the normal oxygen affinity of hemoglobin in human blood depends on the red cell concentration of 2,3-diphosphoglycerate 2,3-DPG, this metabolic intermediate was assayed and oxygen dissociation curves performed on blood stored in ACD and CPD. Further, adenine and inosine were added and their effects on 2,3-DPG concentration and hemoglobin function studied. Hemoglobin function, expressed as p50 the pO2 at which hemoglobin is 50 oxygenated, and 2,3-DPG concentrations were better maintained during the storage period in blood stored in CPD than in ACD. If adenine was present the p50 and 2,3-DPG levels declined more rapidly. However, adenine and inosine in CPD-stored blood allowed the p50 and 2,3-DPG to persist at near normal levels for most of the 3-week storage period. Author
Presented at International Conference on Red Cell Metabolism and Function (1st) Ann Arbor, Mich., 1-3 Oct 69.