It was demonstrated that Venezuelan equine encephalomyelitis VEE virus replicated in and adapted rapidly to human diploid cell strain WI-38. Peak titers of approximately 10 to the 9.8th power mouse intracerebral 50 lethal doses were obtained at low passage levels in Eagles basal medium supplemented with calf serum. VEE virus replicated poorly in serum-free medium. Propagation of VEE virus was accompanied by the production of hemagglutinin and cytopathogenic effects.
Availability: Pub. in Applied Microbiology, v19 n1 p196-198 Jan 1970.