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NEW METHODS FOR EFFECTING WATERTIGHT SEALS ON CORN ROOTS,
NAVAL RADIOLOGICAL DEFENSE LAB SAN FRANCISCO CALIF
Two effective methods of sealing corn roots into capillary glassware are described. One involves only a slight pressure on the root but cannot withstand hydrostatic pressures across the seal in excess of 15 cm of water. The second, capable of withstanding 2 meters of hydrostatic pressure, requires a melted sealing compound which can raise the root epidermis to temperatures between 50 and 56 C for about 2 seconds. Experiments using radioactive tracers have demonstrated that both seals effectively prevent ionic movement external to theroot proper and that the heat-sealing process does not alter the roots absorption-transport capacity. Author