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ELECTROCHEMISTRY OF ENZYMES. DETERMINATION OF PEROXIDASE AND CATALASE.
CHEMICAL RESEARCH AND DEVELOPMENT LABS EDGEWOOD ARSENAL MD
Two representative enzyme systems, peroxide-catalase and peroxide-peroxidase, were used in the development of an electrochemical method for their determination. An electrochemically active substrate was oxidized to two electroinactive products by proper control of the current and experimental conditions. A proton donor with nonelectroactive oxidation and reduction forms was used with peroxidase to evolve its enzymatic function. The donor was not electroactive at the potentials used. With this procedure, 0.0100 to 1.00 unit of peroxidase or catalase was determined with a relative standard deviation of 1.7. The potentials used in the described method were studied by anodic polarographic techniques to determine their significance. Author
Rept. for Jan 63-Jan 64,